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KMID : 0366220050400020065
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Korean Journal of Hematology
2005 Volume.40 No. 2 p.65 ~ p.74
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Eugenol Induces a Reactive Oxygen Species-mediated Apoptosis in HL-60 Human Promyelocytic Leukemia Cells
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Kil Uk-Hyun
Lee Kee-Hyun
Lee Kyung-Tae
Jin Jong-Youl
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Abstract
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Background: Eugenol is a major component of essential oil isolated from the Eugenia caryophyllata (Myrtaceae), which has been widely used as a traditional medicine. In this study, we investigated the effects of eugenol on the cytotoxicity, induction of apoptosis, and the putative pathways of its actions in human promyelocytic leukemia cells (HL-60).
Methods: After applying eugenol on cultured HL-60, changes in the mitochondrial membrane potential were examined by monitoring the cells after double staining with propidium iodide and rhodamin 123 and 2’,7’-Dicholorofluorescin diacetate was used to measure of levels of reactive oxygen species (ROS)
Results: We show that eugenol is a potent inducer of apoptosis and that it transduces the apoptotic signal via ROS generation, thereby inducing mitochondrial permeability transition (MPT) and cytochrome c release to the cytosol. ROS production, mitochondrial alteration, and subsequent apoptotic cell death in eugenol-treated cells were blocked by the antioxidant N-acetylcystein (NAC).
Conclusion: Taken together, the present study demonstrated that eugenol induces the ROS-mediated mitochondrial permeability transition and resultant cytochrome c release.
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KEYWORD
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Eugenol, HL-60 cells, Apoptosis, Reactive oxygen species, Cytochrome c
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